8G2C

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.650, 448.120, 621.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	162.050 - 2.650
R-factor	0.2092
Rwork	0.207
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	162.050	162.050	2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.124	0.023	1.203
Rmeas	0.146	0.027	1.416
Total number of observations	5736096
Number of reflections	1649189	18690	122695
<I/σ(I)>	8.49	31.88	1.02
Completeness [%]	98.7	94.6	99.6
Redundancy	3.478	3.593	3.516
CC(1/2)	0.994	0.999	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME