8G2A

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, peptidyl P-site fMTHSMRC-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.010, 450.380, 625.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.460 - 2.450
R-factor	0.2137
Rwork	0.212
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8cvl
RMSD bond length	0.004
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.460	125.460	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.192	0.059	2.484
Rmeas	0.203	0.062	2.613
Total number of observations	21563296
Number of reflections	2124013	24818	154122
<I/σ(I)>	8.76	27.02	0.86
Completeness [%]	99.2	98.5	97.7
Redundancy	10.152	10.504	10.281
CC(1/2)	0.994	0.992	0.228

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME