8FZK

Dimeric human importin alpha subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	50.107, 90.838, 94.603
Unit cell angles	103.19, 90.37, 97.39

Refinement procedure

Resolution	29.910 - 2.100
R-factor	0.2036
Rwork	0.201
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.387
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.910	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.068	0.527
Rpim	0.042
Number of reflections	92088	8992
<I/σ(I)>	21.41	1.16
Completeness [%]	98.0
Redundancy	3.5
CC(1/2)	0.997	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	14% PEG 8000, 0.2M Magnesium chloride, 0.1M MES