8FZ2
Crystal structure of Fab460 in complex with MPER peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.069, 144.069, 149.445 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.590 - 3.500 |
| R-factor | 0.2642 |
| Rwork | 0.262 |
| R-free | 0.30990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.470 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 3.580 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.231 | 1.158 |
| Rmeas | 0.250 | 1.270 |
| Rpim | 0.094 | 0.510 |
| Number of reflections | 7498 | 482 |
| <I/σ(I)> | 8.67 | 1.25 |
| Completeness [%] | 97.5 | 94.1 |
| Redundancy | 6.7 | 5.7 |
| CC(1/2) | 0.966 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M ammonium phosphate monobasic, 0.1 M Tris-HCl 50% v/v (+/-)-2-Methyl-2,4-pentanediol |
