8FXV
Crystal structure of human proTGF-beta2 in complex with Nb18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03321 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 70.750, 78.160, 88.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.170 - 2.200 |
R-factor | 0.2279 |
Rwork | 0.227 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.753 |
Data reduction software | XDS (20190417) |
Data scaling software | XDS (20190417) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.440 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 25659 | 2538 |
<I/σ(I)> | 10.11 | |
Completeness [%] | 99.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.998 | 0.212 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Crystals of the Nb18/proTGF-beta2 complex (1 microliter) were formed in hanging drops with 1 microliter of 100 mM HEPES pH 7.4, 26.7% Jeffamine ED2001. |