8FXE

Bromodomain of CBP liganded with iCBP6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	35.770, 49.540, 80.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.370 - 1.550
R-factor	0.1575
Rwork	0.156
R-free	0.18640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.694
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.370	1.590
High resolution limit [Å]	1.550	1.550
Rmeas	0.053	0.053
Number of reflections	21029	1290
<I/σ(I)>	25.6	7.5
Completeness [%]	97.8	83.7
Redundancy	9.1	6.3
CC(1/2)	0.999	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.2 M Sodium chloride, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3350