8FX0
Crystal structure of the Trypanosoma cruzi hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRT), isoform D, bound to (S)-SerMe-ImmH Phosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-04-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.199, 80.706, 90.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.080 - 1.520 |
| R-factor | 0.2085 |
| Rwork | 0.208 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc2 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.738 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.080 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.118 | 0.957 |
| Number of reflections | 66957 | 3287 |
| <I/σ(I)> | 13.6 | 4.6 |
| Completeness [%] | 97.1 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M lithium sulfate, 24% PEG3350, 0.1 M HEPES, pH 8.0 |
