8FTO
E. coli 70S ribosome with an improved MS2 tag inserted in H98
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.85 |
| Number of Particle | 2475999 |
| Software | cryoSPARC,PHENIX,SerialEM,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
