8FRR

Wild-type myocilin olfactomedin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.468, 50.527, 50.873
Unit cell angles	90.00, 96.25, 90.00

Refinement procedure

Resolution	27.910 - 1.270
R-factor	0.14
Rwork	0.140
R-free	0.15530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.582
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.910	1.315
High resolution limit [Å]	1.270	1.270
Rmerge	0.088	0.501
Number of reflections	62921	5951
<I/σ(I)>	16.41
Completeness [%]	95.3	91.44
Redundancy	6.4	5.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	5% PEG 8000, 0.05 M magnesium formate