8FR3

E. coli EF-Tu in complex with KKL-55

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	59.385, 65.222, 70.559
Unit cell angles	107.67, 98.79, 107.08

Refinement procedure

Resolution	64.820 - 2.230
R-factor	0.2464
Rwork	0.245
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eze
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.820	2.310
High resolution limit [Å]	2.230	2.230
Rmerge	0.110	0.882
Rmeas	0.121	0.967
Rpim	0.050	0.392
Number of reflections	42498	3782
<I/σ(I)>	9.79
Completeness [%]	94.0	84.31
Redundancy	5.9	5.8
CC(1/2)	0.994	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	30-35% polyethylene glycol monomethyl ether 5,000 (PEG 5K MME), 0.2 M (NH4)2(SO4), 0.1 M MES pH 6.5