8FQ7

Nanobody with WIW inserted in CDR3 loop to Inhibit Growth of Alzheimer's Tau fibrils

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	I 4
Unit cell lengths	78.290, 78.290, 38.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.360 - 1.400
R-factor	0.1913
Rwork	0.189
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.776
Data reduction software	XDS (20190315)
Data scaling software	XSCALE (20190315)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.360	55.360	1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.131	0.115	0.451
Rmeas	0.161	0.141	0.558
Number of reflections	22608	266	1637
<I/σ(I)>	5.25
Completeness [%]	98.5
Redundancy	2.8
CC(1/2)	0.964	0.965	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.15M ammonium sulfate, 0.1 M MES pH 5.5, 25% PEG 4000