8FPX
Crystal structure of tumor related RhoA mutant A161P in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.212, 87.265, 34.044 |
Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
Resolution | 31.570 - 1.470 |
R-factor | 0.1935 |
Rwork | 0.191 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ftn |
RMSD bond length | 0.006 |
RMSD bond angle | 0.930 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.570 | 1.550 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmeas | 0.286 | 1.075 |
Rpim | 0.148 | 0.640 |
Number of reflections | 31430 | 4322 |
<I/σ(I)> | 3 | 1 |
Completeness [%] | 98.9 | 93.4 |
Redundancy | 3.6 | 2.7 |
CC(1/2) | 0.930 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0 |