8FNX
Crystal structure of Hyaluronate lyase B from Cutibacterium acnes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-05-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.676, 103.676, 172.944 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.100 |
R-factor | 0.1577 |
Rwork | 0.156 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.823 |
Data reduction software | XDS (VERSION Nov 1, 2016 BUILT=20170215) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.730 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.172 | 0.672 |
Rmeas | 0.178 | 0.699 |
Rpim | 0.047 | 0.188 |
Number of reflections | 55667 | 7843 |
<I/σ(I)> | 14.5 | 4.3 |
Completeness [%] | 99.5 | 97.8 |
Redundancy | 14.1 | 13.1 |
CC(1/2) | 0.997 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.2 M Na dihydrogen phosphate pH 6.5, 9% PEG 8000, 5 mM TCEP |