8FN8

Crystal structure of the C-terminal Fg domain of TNC with the single FN domain of PTPRZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	54.707, 61.520, 92.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.880 - 1.890
R-factor	0.1672
Rwork	0.164
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2J3F & 1FNA
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.960
High resolution limit [Å]	1.890	1.890
Rmerge	0.113	0.342
Rmeas	0.126	0.384
Rpim	0.054	0.172
Number of reflections	25470	2506
<I/σ(I)>	16.2	4.7
Completeness [%]	99.6	99.2
Redundancy	4.9	4.3
CC(1/2)	0.996	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG3350, 100 mM Tris-HCl, pH 8.0