8FN3
Crystal structure of human DNA polymerase eta incorporating dITP across dC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2022-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 61 |
Unit cell lengths | 98.595, 98.595, 81.376 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.810 - 2.170 |
R-factor | 0.2094 |
Rwork | 0.207 |
R-free | 0.25310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.009 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.910 | 2.210 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 21076 | 1179 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 88.9 | |
Redundancy | 10.9 | |
CC(1/2) | 0.995 | 0.382 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES, pH 6.5, 20% PEG2000 MME, 5 mM calcium chloride |