8FM5

HIV-1 gp120 complex with DY-III-065

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	71.520, 121.660, 195.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.810 - 1.880
R-factor	0.2676
Rwork	0.267
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.388
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.810	2.120
High resolution limit [Å]	1.880	1.880
Rmerge	0.177	1.666
Rpim	0.052	0.514
Number of reflections	88049	4402
<I/σ(I)>	11.9
Completeness [%]	93.7
Redundancy	11.9
CC(1/2)	0.997	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	14% to 16% (w/v) PEG 1500, 0.1 M calcium chloride, 0.1 M imidazole pH 6.5