8FJ6
LSD1-CoREST in complex with T108, long soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.036, 179.360, 233.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.570 - 2.630 |
| R-factor | 0.2195 |
| Rwork | 0.219 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.629 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.570 | 2.680 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.095 | 3.206 |
| Rmeas | 0.108 | 3.705 |
| Rpim | 0.050 | 1.812 |
| Total number of observations | 313206 | 16722 |
| Number of reflections | 73884 | 4376 |
| <I/σ(I)> | 9.9 | 0.5 |
| Completeness [%] | 99.4 | |
| Redundancy | 4.2 | 3.8 |
| CC(1/2) | 0.998 | 0.091 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Na/K Tartrate, 100 mM ADA pH 6.5 |
