8FHM
RNase A-Uridine 5'-Hexaphosphate (RNaseA.p6U)
Refinement Statistics
Experimental method: SOLUTION SCATTERING, X-RAY DIFFRACTION (1.79 Å)
Cell axes | 100.069 | 32.527 | 72.785 |
Cell angles | 90.00 | 90.48 | 90.00 |
Spacegroup | C 1 2 1 | ||
Resolution limits | 30.93 - 1.79 |
Crystallization Conditions
crystal ID | method | pH | pH range | temperature | unit |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 |
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-10-23 |
Detector | APEX II CCD |
Wavelength(s) | 1.544178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.069, 32.527, 72.785 |
Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
Resolution | 30.930 - 1.790 |
R-factor | 0.1808 |
Rwork | 0.178 |
R-free | 0.23090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1afu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.384 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.930 | 1.860 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 22299 | 22299 |
<I/σ(I)> | 3.13 | |
Completeness [%] | 99.3 | |
Redundancy | 17.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 25% PEG4000, 20 mM citrate, pH 6, crystal soaked in 50 mM ligand, 30% PEG4000, 25% glycerol, 20 mM citrate |