8FG0

Crystal structure of the 3764 Fab in complex with the C-terminal PfCSP linker, PfCSP281-294.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 1 21 1
Unit cell lengths	92.236, 66.701, 95.540
Unit cell angles	90.00, 113.41, 90.00

Refinement procedure

Resolution	39.210 - 2.360
R-factor	0.1782
Rwork	0.175
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.929
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.460
High resolution limit [Å]	2.360	2.360
Number of reflections	43719	5067
<I/σ(I)>	10.9
Completeness [%]	98.9
Redundancy	6.9
CC(1/2)	0.997	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	19% (v/v) isopropanol, 19% (w/v) PEG 4000, 5% (v/v) glycerol, 0.095 M sodium citrate, pH 5.6