8FFM
Wildtype rat TRPV2 in nanodiscs bound to RR and 2-APB
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.9 |
Number of Particle | 690077 |
Software | Topaz,cryoSPARC,ISOLDE,CTFFIND,RELION |
Symmetry | C4 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |