8FE6

Crystal structure of human O-GlcNAc transferase (OGT) in complex with an exosite-binding peptide and UDP-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.127129
Spacegroup name	H 3
Unit cell lengths	281.532, 281.532, 143.212
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.640 - 3.060
R-factor	0.1816
Rwork	0.181
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.532
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.485	3.113
High resolution limit [Å]	3.060	3.060
Number of reflections	79618	3990
<I/σ(I)>	12.9
Completeness [%]	99.7
Redundancy	10.5
CC(1/2)	0.994	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.45M potassium phosphate, 10mM EDTA pH 8, 1% Xylitol