8FE6
Crystal structure of human O-GlcNAc transferase (OGT) in complex with an exosite-binding peptide and UDP-GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.127129 |
| Spacegroup name | H 3 |
| Unit cell lengths | 281.532, 281.532, 143.212 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.640 - 3.060 |
| R-factor | 0.1816 |
| Rwork | 0.181 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.532 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.485 | 3.113 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Number of reflections | 79618 | 3990 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.994 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.45M potassium phosphate, 10mM EDTA pH 8, 1% Xylitol |
