8FC6

Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.35A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.600, 449.650, 619.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	181.990 - 2.350
R-factor	0.2127
Rwork	0.211
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.004
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	181.990	181.990	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.206	0.067	2.771
Rmeas	0.223	0.072	2.986
Total number of observations	16536950
Number of reflections	2388473	27834	176217
<I/σ(I)>	6.95	20.97	0.82
Completeness [%]	99.8	99.1	99.9
Redundancy	6.924	7.037	7.076
CC(1/2)	0.992	0.993	0.203

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME