8FBK
Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 70.746, 70.746, 200.499 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.270 - 3.150 |
| R-factor | 0.2709 |
| Rwork | 0.267 |
| R-free | 0.30880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.346 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.270 | 3.370 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.266 | 2.286 |
| Number of reflections | 5682 | 12073 |
| <I/σ(I)> | 6 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.3 | 12.1 |
| CC(1/2) | 0.995 | 0.648 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M magnesium chloride, 0.1 M imidazole, pH 8.0, 35% v/v MPD |
