8FBH

Crystal structure of NSD1 Mutant-T1927A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	66.293, 67.382, 70.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.170 - 2.320
R-factor	0.1908
Rwork	0.188
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ooi
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.360
High resolution limit [Å]	2.320	6.290	2.320
Rmerge	0.111	0.054	0.937
Rmeas	0.119	0.057	1.035
Rpim	0.042	0.020	0.428
Total number of observations	110550
Number of reflections	13958	782	695
<I/σ(I)>	8.2
Completeness [%]	99.8	98.7	99.7
Redundancy	7.9	7.6	5.7
CC(1/2)		0.998	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.1M NH4SO4, 0.2M NaCl, 0.1M Hepes pH