8FBG

Crystal structure of NSD1 Mutant-Y1869C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-12
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	42.449, 64.947, 81.408
Unit cell angles	90.00, 93.17, 90.00

Refinement procedure

Resolution	27.090 - 2.290
R-factor	0.217
Rwork	0.214
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ooi
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.290	6.240	2.300
Rmerge	0.113	0.068	0.771
Rmeas	0.128	0.077	0.881
Rpim	0.061	0.035	0.419
Number of reflections	19253	1027	975
<I/σ(I)>	10.8
Completeness [%]	96.7	98.6	96.8
Redundancy	4.1	4.1	4
CC(1/2)		0.995	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% p3350, 0.2M KCl