8F8Y

PHF2 (PHD+JMJ) in Complex with VRK1 N-Terminal Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	74.406, 84.065, 105.215
Unit cell angles	90.00, 103.99, 90.00

Refinement procedure

Resolution	38.870 - 3.060
R-factor	0.1972
Rwork	0.195
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.372
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.870	3.160
High resolution limit [Å]	3.060	3.060
Rmerge	0.187	0.795
Rpim	0.136	0.624
Number of reflections	20652	1755
<I/σ(I)>	4.4	0.8
Completeness [%]	86.3	74.3
Redundancy	2.6
CC(1/2)	0.967	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	292	2.5M Ammonium Sulfate 90mM Bis-Tris Propane, pH 6.3 4% Pentaerythritol ethoxylate (3/4 EO/OH)