8F6B
Crystal structure of murine PolG2 hexamer bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2017-10-19 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 116.224, 116.224, 250.938 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.040 - 2.750 |
R-factor | 0.1966 |
Rwork | 0.196 |
R-free | 0.22150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g5i |
RMSD bond length | 0.002 |
RMSD bond angle | 0.426 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.040 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmeas | 0.096 | 1.075 |
Number of reflections | 98810 | 9883 |
<I/σ(I)> | 18.96 | 1.08 |
Completeness [%] | 99.8 | 99.81 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% MPD 0.1 M MES 2:1 protein to DNA ratio |