8F6B

Crystal structure of murine PolG2 hexamer bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	105
Detector technology	CCD
Collection date	2017-10-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	116.224, 116.224, 250.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.040 - 2.750
R-factor	0.1966
Rwork	0.196
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g5i
RMSD bond length	0.002
RMSD bond angle	0.426
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.040	2.850
High resolution limit [Å]	2.750	2.750
Rmeas	0.096	1.075
Number of reflections	98810	9883
<I/σ(I)>	18.96	1.08
Completeness [%]	99.8	99.81
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10% MPD 0.1 M MES 2:1 protein to DNA ratio