8F5U

Rabbit muscle pyruvate kinase in complex with magnesium, potassium and pyruvate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	113.131, 130.198, 170.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.270 - 2.300
R-factor	0.1667
Rwork	0.164
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g50
RMSD bond length	0.006
RMSD bond angle	0.746
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.380
High resolution limit [Å]	2.300	4.960	2.300
Rmerge	0.140	0.061	0.541
Total number of observations	810287
Number of reflections	216125	21594	21593
<I/σ(I)>	7.8
Completeness [%]	100.0	99.8	100
Redundancy	3.7	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	282	50 mM Tris 50 mM NaCl and a range of 7.5 to 10% PEG 20,000