8F3K
Anti-CRISPR protein AcrIIC5 inhibits CRISPR-Cas9 by acting as a DNA mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 105 |
| Detector technology | PIXEL |
| Collection date | 2018-08-03 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.622, 46.105, 69.974 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.500 - 2.100 |
| R-factor | 0.2097 |
| Rwork | 0.204 |
| R-free | 0.25440 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.791 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.20.1-4487) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 38.500 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.082 |
| Number of reflections | 14852 | 8432 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 96.8 | 96.77 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris pH 8.5 and 2.0M ammonium sulfate |
