8F2V

Antibody Fab directed against SARS-CoV-2 Spike Protein Receptor Binding Domain (RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	110.011, 184.182, 181.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 3.500
R-factor	0.2486
Rwork	0.246
R-free	0.28890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5anm
RMSD bond length	0.003
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.670	3.580
High resolution limit [Å]	3.490	3.490
Number of reflections	23601	1632
<I/σ(I)>	9	1.5
Completeness [%]	99.3
Redundancy	9.1
CC(1/2)	0.994	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 8000, 15 %, 0.1 M MES, 0.2 M calcium acetate