8F24
Mirror-image RNA octamer containing 2'-OMe-L-uridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2022-10-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | H 3 2 |
Unit cell lengths | 47.070, 47.070, 365.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.940 - 3.200 |
R-factor | 0.2215 |
Rwork | 0.213 |
R-free | 0.29824 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kw4 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.501 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.310 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.074 | 0.874 |
Rmeas | 0.077 | 0.903 |
Rpim | 0.021 | 0.224 |
Number of reflections | 2795 | 709 |
<I/σ(I)> | 30.1 | 4.2 |
Completeness [%] | 98.5 | 99.7 |
Redundancy | 8.2 | 8.4 |
CC(1/2) | 0.983 | 0.935 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium sulfate, 0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400 |