7KW4

L-DNA containing 2'-fluoro modification

Summary for 7KW4

• Entry DOI: 10.2210/pdb7kw4/pdb
• Descriptor: DNA (5'-D(*(0DG)P*(0FU)P*(0DG)P*(0DT)P*(0DA)P*(0DC)P*(0DA)P*(0DC))-3'), MAGNESIUM ION (3 entities in total)
• Functional Keywords: l-dna, fluoro modification, dna
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 2438.89

Authors

Zhang, W.,Zhang, Y. (deposition date: 2020-11-29, release date: 2021-09-01, Last modification date: 2023-10-18)

Primary citation

Dantsu, Y.,Zhang, Y.,Zhang, W.
Synthesis and Structural Characterization of 2'-Deoxy-2'-fluoro-l-uridine Nucleic Acids.
Org.Lett., 23:5007-5011, 2021

PubMed Abstract: Despite the development of artificial l-RNA/DNA as therapeutic molecules, the in-depth investigation on their chemical modifications is still limited. Here, we synthesize a chemically derivatized 2'-deoxy-2'-fluoro-l-uridine building block and incorporate it into oligonucleotides. Our thermo-denaturization and enzymatic digestion experiments reveal their superior stability. Furthermore, one crystal structure of l-type fluoro-DNA is determined to characterize its handedness. Our results reveal the increase of l-helix stability by fluoro-modification and provide the foundation for its future functional application.

PubMed: 34142829
DOI: 10.1021/acs.orglett.1c01498

Experimental method

X-RAY DIFFRACTION (1.03 Å)