8EZK
Pfs25 in complex with transmission-reducing antibody AS01-04
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.250, 91.720, 122.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.060 - 2.300 |
R-factor | 0.2308 |
Rwork | 0.229 |
R-free | 0.26020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6phb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.804 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.060 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.043 | 0.561 |
Number of reflections | 34702 | 3380 |
<I/σ(I)> | 10.3 | 1.23 |
Completeness [%] | 99.8 | 98.66 |
Redundancy | 7.9 | 5.1 |
CC(1/2) | 0.997 | 0.694 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M potassium thiocyanate, 23% PEG 3350. Cryoprotected in 30% MPD |