8EZ6

The DBC1/SIRT1 Interaction is Choreographed by Post-translational Modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-12-04
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	37.388, 50.822, 62.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.320 - 2.000
R-factor	0.2053
Rwork	0.202
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wfq
RMSD bond length	0.010
RMSD bond angle	1.277
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.320	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.049	0.127
Number of reflections	8285	602
<I/σ(I)>	37.5
Completeness [%]	97.9	96.3
Redundancy	13.59	13.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		298	0.1M sodium acetate pH 4.6, 2M ammonium sulfate