8EW6
Anti-human CD8 VHH complex with CD8 alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-10-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00001 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 57.115, 83.554, 51.302 |
Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
Resolution | 32.481 - 1.904 |
R-factor | 0.2024 |
Rwork | 0.200 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NULL |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.150 | 1.900 |
Rmerge | 0.048 | 0.041 | 0.254 |
Rmeas | 0.058 | 0.049 | 0.316 |
Rpim | 0.032 | 0.027 | 0.186 |
Total number of observations | 48809 | ||
Number of reflections | 16891 | 956 | 636 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 90.9 | 99.9 | 69.5 |
Redundancy | 2.9 | 3.3 | 2.4 |
CC(1/2) | 0.994 | 0.869 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 15% PEG 3350 and 0.1M succinic acid pH 7.0 |