8EUS
Crystal structure of NPC1 luminal domain C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.145, 65.207, 68.534 |
Unit cell angles | 90.00, 91.69, 90.00 |
Refinement procedure
Resolution | 41.190 - 2.300 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f18 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.646 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.120 | 0.600 |
Rmeas | 0.130 | 0.680 |
Rpim | 0.053 | 0.300 |
Number of reflections | 20421 | 1522 |
<I/σ(I)> | 13.1 | 3.1 |
Completeness [%] | 98.1 | 97.4 |
Redundancy | 5.5 | 4.8 |
CC(1/2) | 0.998 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M sodium malonate 20% PEG 3,350 |