8EQE

Co-crystal structure of PERK with compound 26

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	125.342, 125.342, 58.084
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.275 - 2.559
Rwork	0.224
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x7j
RMSD bond length	0.003
RMSD bond angle	1.249
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.275	2.630
High resolution limit [Å]	2.559	2.559
Rmerge	0.072	1.180
Number of reflections	17214	1254
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4% tacsimate pH 7.0 and 12% PEG 3350