8EQ5
Crystal structure of the N-terminal kinase domain of RSK2 in complex with SPRED2 (131-160)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-16 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 83.438, 41.105, 99.258 |
Unit cell angles | 90.00, 113.87, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.800 |
R-factor | 0.185 |
Rwork | 0.183 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gue |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.390 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.771 |
Rpim | 0.057 | 0.752 |
Number of reflections | 28136 | 1656 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 97.7 | |
Redundancy | 3.3 | |
CC(1/2) | 0.990 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 30% w/v PEG 2K MME, 0.1 M Tris, pH 8.0 |