8EOO
Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing human antibodies WRAIR-2063 and WRAIR-2151
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-23 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.066, 97.409, 141.129 |
| Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.770 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7n4m |
| RMSD bond angle | 15.178 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.090 | 2.830 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.140 | 1.313 |
| Rmeas | 0.151 | |
| Rpim | 0.057 | 0.592 |
| Number of reflections | 65626 | 3949 |
| <I/σ(I)> | 11 | 1 |
| Completeness [%] | 98.7 | 86.1 |
| Redundancy | 6.9 | 5.4 |
| CC(1/2) | 0.996 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Bis-Tris propane HCl pH 8.5, 200 mM Na malonate dibasic, 20% w/v PEG 3350 |
