8EMW
Phospholipase C beta 3 (PLCb3) in complex with Gbg on liposomes
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Number of Particle | 1997424 |
Software | crYOLO,cryoSPARC,RELION,PHENIX,SerialEM,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |