8EM0

HRAS R97V Crystal Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	H 3 2
Unit cell lengths	89.146, 89.146, 135.545
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.030 - 2.109
Rwork	0.156
R-free	0.20090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k8y
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.990
High resolution limit [Å]	1.770	4.800	1.950
Rmerge	0.174	0.064
Rmeas	0.186	0.070
Rpim	0.085	0.030
Total number of observations	132530
Number of reflections	27487	1975	615
<I/σ(I)>	3.9
Completeness [%]	69.9	100	31.3
Redundancy	4.8	5.6	1.2
CC(1/2)		0.998	0.047

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT