8ELS
HRAS R97A Crystal Form 1 R-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.856, 88.856, 134.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.709 - 2.267 |
| R-factor | 0.1671 |
| Rwork | 0.162 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 2.310 |
| High resolution limit [Å] | 2.267 | 6.150 | 2.270 |
| Rmerge | 0.143 | 0.039 | 0.945 |
| Number of reflections | 9740 | 531 | 470 |
| <I/σ(I)> | 5.1 | ||
| Completeness [%] | 99.9 | 99.1 | 99.6 |
| Redundancy | 10.3 | 10.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 3350, Calcium Acetate, HEPES, Calcium Chloride, Magnesium Chloride |
