8ELS

HRAS R97A Crystal Form 1 R-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-02-12
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	88.856, 88.856, 134.953
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.709 - 2.267
R-factor	0.1671
Rwork	0.162
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k8y
RMSD bond length	0.008
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.310
High resolution limit [Å]	2.267	6.150	2.270
Rmerge	0.143	0.039	0.945
Number of reflections	9740	531	470
<I/σ(I)>	5.1
Completeness [%]	99.9	99.1	99.6
Redundancy	10.3	10.1	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	PEG 3350, Calcium Acetate, HEPES, Calcium Chloride, Magnesium Chloride