8EKH

I-2 Y35N H35N (unbound) Fab from CH65-CH67 lineage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	127.724, 70.652, 75.759
Unit cell angles	90.00, 119.35, 90.00

Refinement procedure

Resolution	39.410 - 2.700
R-factor	0.2486
Rwork	0.244
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hk0
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.700	2.700
Number of reflections	15770	2360
<I/σ(I)>	7.39	2.08
Completeness [%]	96.4	90.8
Redundancy	5.5
CC(1/2)	0.978	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.8M lithium sulfate monohydrate, 0.1M sodium acetate trihydrate (pH 4), 4% polyethylene glycol 200