8EKF
X-ray crystal structure of 311R Fab in complex with the PfCSP peptide NPNA-3
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.412, 70.765, 170.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.270 - 1.900 |
Rwork | 0.184 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6axk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.718 |
Data reduction software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.270 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | |
Number of reflections | 44216 | 4800 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 99.8 | 97.6 |
Redundancy | 3.3 | 2.3 |
CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.04 M KH2PO4, 20% Glycerol, and 16% PEG3000 |