8EK8

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAAAGGAGAAGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000050
Spacegroup name	P 21 21 21
Unit cell lengths	44.058, 61.064, 154.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.810 - 2.630
R-factor	0.241
Rwork	0.237
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.810	2.724
High resolution limit [Å]	2.630	2.630
Rmerge	0.075	0.936
Rmeas	0.031	0.374
Rpim	0.081	1.000
Number of reflections	12962	1263
<I/σ(I)>	17.17	2.43
Completeness [%]	98.7	99.21
Redundancy	7	7.1
CC(1/2)	0.999	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	200 mM Ammonium Acetate, 100 mM TRIS, pH=8.5, 25% PEG 3350