8EK6
UCA Y35N (unbound) Fab from CH65-CH67 lineage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.547, 70.707, 86.934 |
Unit cell angles | 90.00, 126.96, 90.00 |
Refinement procedure
Resolution | 49.760 - 1.954 |
Rwork | 0.185 |
R-free | 0.21590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hk0 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.900 | 1.950 |
Rmerge | 0.356 | |
Rmeas | 0.052 | 0.503 |
Number of reflections | 43266 | 6868 |
<I/σ(I)> | 17.23 | 1.93 |
Completeness [%] | 98.3 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M succinic acid (pH 7), 0.1 M bicine (pH 8.5), 30% v/v polyethylene glycol monomethyl ether 550 |