8EJP

Crystal structure of the homeodomain of Platypus sDUX in complex with DNA containing 5-Bromouracil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	74.499, 74.499, 99.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.676 - 2.174
R-factor	0.2397
Rwork	0.238
R-free	0.25930
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.676	59.676	2.329
High resolution limit [Å]	2.174	7.091	2.174
Rmerge	0.112	0.091	1.444
Rmeas	0.117	0.095	1.504
Rpim	0.032	0.026	0.416
Total number of observations	139973	6026	6739
Number of reflections	10466	523	523
<I/σ(I)>	10.7	21	1.5
Completeness [%]	94.4	99.6	64.5
Redundancy	13.4	11.5	12.9
CC(1/2)	0.998	0.997	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3350