8EJ8
Human PU.1 ETS-Domain (165-270) Q226E Mutant Bound to d(AATAAAAGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.960, 60.611, 44.659 |
Unit cell angles | 90.00, 116.73, 90.00 |
Refinement procedure
Resolution | 33.320 - 1.450 |
R-factor | 0.1383 |
Rwork | 0.136 |
R-free | 0.17160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.320 | 1.502 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.051 | 0.464 |
Rmeas | 0.061 | 0.549 |
Rpim | 0.033 | 0.290 |
Number of reflections | 34842 | 3396 |
<I/σ(I)> | 13.9 | 2.38 |
Completeness [%] | 95.8 | 94.12 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.997 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |