8EIM

Structure of ALAS with C-terminal truncation from S. cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	79.280, 99.550, 120.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.909 - 2.100
Rwork	0.188
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5txr
RMSD bond length	0.007
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.910	2.220
High resolution limit [Å]	2.100	2.100
Rmeas	0.119	1.714
Number of reflections	56191	8843
<I/σ(I)>	11.8	1.27
Completeness [%]	99.7	98.5
Redundancy	6.5
CC(1/2)	0.998	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	0.2M magensium acetate, 20% PEG 3350