8EIA

Crystal structure of beta-catenin and the MDM2 p53-binding domain in complex with H333, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 1 21 1
Unit cell lengths	49.810, 96.066, 76.790
Unit cell angles	90.00, 106.62, 90.00

Refinement procedure

Resolution	47.730 - 3.600
R-factor	0.3555
Rwork	0.354
R-free	0.37270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uwi
RMSD bond length	0.003
RMSD bond angle	0.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	3.940
High resolution limit [Å]	3.600	3.600
Rmerge	0.241	0.912
Number of reflections	7517	1803
<I/σ(I)>	5.3	1.7
Completeness [%]	92.3	94.29
Redundancy	5.5	5.4
CC(1/2)	0.978	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M Potassium chloride, 0.1 M HEPES pH 7, 15% w/v PEG 5000 MME